A Validated Reverse Phase Hplc Method for the Simultaneous Estimation of Artemether and Lumefantrine in Pharmaceutical Dosage Forms
نویسندگان
چکیده
A simple, sensitive and precise reverse phase high performance liquid chromatographic method has been developed for the simultaneous estimation of Artemether and Lumefantrine in pharmaceutical dosage forms. The mobile phase consisted of Acetonitrile: buffer (0.1% v/v ortho phosphoric acid, PH – 3) in the ratio of 60:40 v/v delivered at a flow rate of 1.5 ml / min and wavelength of detection at 303 nm. The retention times of Artemether and Lumefantrine were 13.888 min and 7.207 min respectively. The developed method was validated according to ICH guidelines. The proposed method can be used for determination of these drugs in combined dosage forms.
منابع مشابه
Validated Reverse Phase High Performance Liquid Chromatographic Method for the Simultaneous Determination of Artemether and Lumefantrine in Fixed Combined Dosage Form
Objective: The present aim is to develop simple, precise, and accurate reverse phase high performance liquid chromatographic method (RP-HPLC) for the simultaneous assay of artemether and lumefantrine in fixed combined dosage form. Methods: The chromatographic study was carried out on Hypersil C18 column (250×4.6 mm, 5 μ) with mobile phase containing a mixture of KH2PO4 buffer (pH-3.5) and aceto...
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